This review analyzes the impact of knowledge management (KM) and information systems (IS) on knowledge ambidexterity and demonstrates how organizations can achieve knowledge ambidexterity. We conduct a systematic literature review of 65 journal articles from scholarly databases, from which we identify the antecedents of knowledge ambidexterity. Based on our critical review, we create a conceptual framework to achieve knowledge ambidexterity. Subsequently, we discuss the evolution of IS and its relationship with knowledge ambidexterity to demonstrate how organizations can achieve knowledge ambidexterity. The review shows a lack of studies that examine how firms can leverage the benefits of KM and IS to achieve knowledge ambidexterity. We propose that the combination of exploration and exploitation processes can achieve knowledge ambidexterity. Our framework proposes the use of contemporary IS tools, such as Big Data, to balance the knowledge exploration and exploitation processes. These recommendations have implications for promoting knowledge ambidexterity in organizations.